TOOLSee Saliva Colorimetric RT-LAMP kit
The TOOLSee Saliva Colorimetric RT-LAMP kit offers simple, time-saving, and affordable method including saliva extraction and direct RT-LAMP reaction to detect target gene in form of RNA from saliva via reverse transcription loop-mediated isothermal amplification (RT-LAMP). Saliva is an excellent and non-invasive source of RNA for rapid testing. The saliva extraction is suitable and stable for nucleic acids analysis by simple mix and heating. The extraction is able to be subjected to following analysis by RT-LAMP reactions contains optimized indicator to the RT-LAMP reaction master mix without inhibiting amplification efficiency. The reducing concentration of Mg+2 in RT-LAMP reaction leading to a color change from violet to sky blue. This Saliva Colorimetric RT-LAMP kit can be used for saliva extracted sample and applies to detection of virus and micro pathogens. The end amplicon of TOOLSee Colorimetric RT-LAMP kit can be verified by agarose gel electrophoresis.T
Features
Workflow
- Fast: direct extraction of saliva can be used for experiments.
- Simple: easy to operate, just simple mixing and heating.
- Intuitive: you can directly observe the results without instruments, the color will change from violet to sky blue after RT-LAMP reaction.
Workflow
RT-LAMP of human RNase P RNA from human saliva extracted with TOOLS Quick Saliva Extraction Reagent
- 1, 2, 3 :Saliva samples were collected from three individuals
- NC: Negative Control-extraction buffer as template
- PC: Positive Control-RNase P RNA oligo as template
Reverse transcription loop-mediated isothermal amplification of human RNase P RNA from human saliva extracted with TOOLS Quick Saliva Extraction Reagent. The reaction tubes were Incubated at 63°C for 30 minutes. TOOLSee negative reactions are indicated in violet and positive reactions are indicated by a change to sky blue.